Very happy to see our paper on uncovering a novel candidate gene (Kinesin-12) associated with the resistance to the Bt toxin Cry1Ac in the agricultural pest Helicoverpa zea is out in GENETICS (https://doi.org/10.1093/genetics/iyac037). This paper had it all, a de novo chromosome level assembly of H. zea, bulk segregant analysis of Cry1Ac resistance followed by fine-scale mapping, RNAseq analysis of H. zea guts to support identification of candidate, and population analysis. This lead to the identification of Kinesin-12, a gene not previously linked to Bt toxin resistance, as a significant contributor to Cry1Ac resistance in this important agricultural pest. This was a wonderful collaborative project lead by lab postdoc, Dr. Kyle Benowitz (currently an Assistant Professor at Austin Peay State University). Lab manager Carson Allan was also instrumental in the success of the project. This project would not have been possible without the other members of the Helicoverpa team, Dr. Bruce Tabashnik (UA Entomology), Dr. Yves Carriere (UA Entomology), Dr. Jeff Fabrick (USDA ARS), Dr. Xianchun Li (UA Entomology), Ben Degain (Carriere lab).
You can listen to it here: https://media.kjzz.org/s3fs-public/covid-science-shutdown-revisited-ng-20220117.mp3
No fly pictures this time, but a beautiful rotten organ pipe cactus is shown (if only we had smell-o-vision).
It was a wonderful and productive fours years and although I new this moment will eventually happen, it is sad to see postdocs Kyle Benowitz and Fernando Diaz leave the lab. I am very proud of them for all the success and work they have achieved while in the lab at the University of Arizona. This is especially the case given all that they had to deal with over the last year and a half with the COVID-19 pandemic. It was a tough year to be looking for academic jobs, but they persevered. And although we will still be collaborating on projects and manuscripts for sometime, I will miss their presence and impact on my lab. Kyle is heading to Austin Peay State University as an Assistant Professor in the Department of Biology, and Fernando will be a Visiting Assistant Professor of Biology at Colgate University. Wishing them the very best, much success and happiness.
Our lab was featured in a story about getting science done during a pandemic. On the written story you can see a picture of Fernando Diaz (postdoc) in the field in Organ Pipe National Monument, a picture of some of our fly cages taken by Carson Allan (lab manager), and the reference to being in the lab around the clock was to the experiments that Kyle Benowitz (postdoc) did.
On June 15, 2020 we officially started our work on uncovering the genetics of Bt resistance in the agricultural pest Helicoverpa zea (Corn earworm, CEW). This work is in collaboration with friends and great colleagues from the Department of Entomology at the University of Arizona, Bruce Tabashnik and Yves Carriere, and from the USDA ALARC, Jeff Fabrick.
Today Tierney Shaible did a wonderful job in presenting her impressive amount of work on D. mojavensis and how they adjust at the behavioral, life history, physiological and transcription level to the summer conditions of the Sonoran Desert.
Stay tuned for the two manuscripts that will come out of her Thesis work.
Working on ecologically interesting species is a powerful way to link patterns of variation from genes/genomes to organism level traits to the selective forces shaping it. To facilitate this, having a chromosome-level genome assembly is very helpful. In this paper we outline how this is an attainable goal. We provide a step-by-step guide for investigators that work on ecologically exciting species, but do not have a vast computational background.
Happy to see our BMC Genomics paper out. This is the first ever paper that examines the pattern of genomic evolution across the four cactus host populations of Drosophila mojavensis. So glad to see all of Carson’s hard work finally in print.
Allan, C. W. and Matzkin, L. M. (2019). Genomic analysis of the four ecologically distinct cactus host populations of Drosophila mojavensis. BMC Genomics. 20(1):732. doi:10.1186/s12864-019-6097-z
Our paper looking at the underlying genetic architecture of locomotor behavior in Drosophila mojavensis using bulk segregant analysis is out in G3 Early Online. http://www.g3journal.org/content/early/2019/03/29/g3.119.400036. Kyle Benowitz and Josh Coleman put a tremendous amount of effort in this study. Awesome work.
Very excited to say that we have several other exciting studies on the genetic architecture of behavior, life history and morphological traits in D. mojavensis coming!!!
Very happy to be notified that our NIH proposal on the genetic basis of infertility in collaboration with Jeremy Bono (University of Colorado, Colorado Springs) will get funded.